10-Plex HCR™ Gold Spectral Imaging
Robust 10-Plex RNA and Protein Imaging
HCR™ Gold RNA-FISH/IF enables robust imaging of 10 RNA and/or protein targets with 1-step quantitative HCR™ Gold signal amplification for all 10 targets simultaneously
No Repeated Imaging
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Suitable even for whole-mounts and delicate specimens that are not amenable to repeated imaging
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No repeated staining, imaging, stripping, or registration
Standardized Ingredients for Robust Performance
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HCR™ Gold Amplifiers: orthogonal 10-set
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Labels: optimized 10-set
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Excitation wavelengths: optimized 10-set
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Detection wavelengths: optimized 10-set
Quantitative in All 10 Channels
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All 10 channels enable analog RNA or protein relative quantitation with subcellular resolution
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All 10 channels enable digital RNA absolute quantitation with single-molecule resolution
10-Plex HCR™ Gold RNA-FISH/IF with Spectral Imaging and Linear Unmixing
We recommend labels (405, 425, 488, 514, 546, 594, 633, 700, 750, 800) for robust 10-plex HCR™ Gold spectral imaging. Linear unmixing yields one fluorescent channel per fluorophore plus one channel for autofluorescence.
A new era for in situ hybridization:
multiplex, quantitative, high-resolution, sensitive, versatile, robust
Experience multiplex, quantitative, high-resolution immunfluorescence: amplified, enzyme-free, FFPE, simple
Quantitative 1-step enzyme-free HCR™ Gold signal amplification for all RNA and protein targets simultaneously