Infinite Catalog
HCR™ HiFi Probes enable multiplex quantitative RNA-FISH (RNA fluorescence in situ hybridization), RNA flow cytometry, and northern blotting with automatic background suppression throughout the protocol.
For organisms in our Infinite Catalog, when you place your order, you will see suggested target matches as you type. For new organisms, you can provide a gene symbol and target sequence.
Whether your target is already in our Infinite Catalog or just joining it, HCR™ HiFi Probes for any target RNA in any organism across the tree of life (view gallery) are designed by the MI Team using MI’s proprietary probe design process with no design fee.
All HCR™ HiFi Probes in our Infinite Catalog are backed by the HCR™ HiFi Probe Promise.
Simple Robust Protocols
Protocols for HCR™ Gold RNA-FISH, HCR™ Gold RNA Flow Cytometry, and HCR™ Gold Northern Blots are simple, robust, and enzyme-free, requiring only 2 stages independent of the number of target RNAs.
Automatic Background Suppression
HCR™ HiFi Probes and HCR™ Gold Amplifiers provide automatic background suppression throughout the protocol, ensuring that even if probes or amplifiers bind non-specifically in the sample they will not generate amplified background, dramatically enhancing performance and ease-of-use.
Straightforward Multiplexing
HCR™ Gold Amplifiers enable straightforward multiplexing using 1-step quantitative signal amplification for all target RNAs simultaneously.
Image Any mRNA. In Any Organism. In Any Sample Type.
HCR™ Gold RNA-FISH offers unmatched versatility (see gallery): FFPE tissue sections, fresh/fixed frozen tissue sections, thick brain slices, cells in solution or on a slide, whole-mounts, cleared samples, model organisms, non-model organisms, environmental samples, or multi-kingdom samples.
Quantitative RNA Analysis Modes
HCR™ HiFi Probes enables four quantitative analysis modes for RNA targets:
Subcellular Quantitative RNA Imaging
Subcellular HCR™ Gold RNA-FISH enables analog RNA relative quantitation with subcellular resolution in the anatomical context of thick autofluorescent samples.
Single-Molecule Quantitative RNA Imaging
Single-molecule HCR™ Gold RNA-FISH enables digital RNA absolute quantitation with single-molecule resolution in the anatomical context of thick autofluorescent samples.
Quantitative RNA Flow Cytometry
Quantitative HCR™ Gold RNA Flow Cytometry enables analog RNA relative quantitation for high-throughput expression profiling of mammalian cells and bacteria.
Quantitative Northern Blots
Quantitative HCR™ Gold Northern Blots enable simultaneous quantification of RNA target size and abundance.
A new era for in situ hybridization:
multiplexed, quantitative, high-resolution, sensitive, versatile, robust
RNA relative quantitation for high-throughput expression profiling of mammalian cells and bacteria
