HCR™ Gold Northern Blots
Multiplex quantitative RNA blotting
HCR™ Gold RNA-FISH kits enable multiplex quantitative northern blots for simultaneous quantification of RNA target size and abundance with automatic background suppression throughout the protocol
Simple Robust Protocols
HCR™ Gold Northern Blot protocols are simple, robust, and enzyme-free, requiring only 2 stages (probe hybridization, amplification) independent of the number of target RNAs
RNA Relative Quantitation via HCR™ Northern Blot
Relative Quantitation
For a given target, relative quantitation is performed by comparing band intensities between lanes.
3-Plex RNA Relative Quantitation
High Accuracy and Precision
Standard curves demonstrate high accuracy (linearity with zero intercept) and precision (tight scatter around the line).
RNA Absolute Quantitation via HCR™ Northern Blot
Absolute Quantitation
Absolute quantitation is performed by comparing samples to a standard curve generated using RNA targets of known abundance.
Absolute miRNA Quantitation for HEK 293T and HeLa Cells
High Accuracy and Precision
Standard curves demonstrate high accuracy (linearity with zero intercept) and precision (tight scatter around the line).
Automatic Background Suppression
HCR™ Gold Northern Blots provide automatic background suppression throughout the protocol, ensuring that even if probes or amplifiers bind non-specifically in the sample they will not generate amplified background, dramatically enhancing performance and ease-of-use.
Straightforward Multiplexing
HCR™ Gold Northern Blots enable straightforward multiplexing using 1-step quantitative signal amplification for all target RNAs simultaneously.
Infinite Catalog
Whether your target is already in our Infinite Catalog or just joining it, HCR™ HiFi Probes for any target RNA in any organism across the tree of life are designed by the MI Team using MI’s proprietary probe design process with no design fee.
Quantitative Signal
HCR™ Gold Northern Blot signal is quantitative in the form of band intensities that scale approximately linearly with target abundance.