HCR™ Gold Northern Blots

Multiplex quantitative RNA blotting

HCR™ Gold RNA-FISH kits enable multiplex quantitative northern blots for simultaneous quantification of RNA target size and abundance with automatic background suppression throughout the protocol

Simple Robust Protocols

HCR™ Gold Northern Blot protocols are simple, robust, and enzyme-free, requiring only 2 stages (probe hybridization, amplification) independent of the number of target RNAs

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Safety Data Sheets

RNA Relative Quantitation via HCR™ Northern Blot

Relative Quantitation

For a given target, relative quantitation is performed by comparing band intensities between lanes.

3-Plex RNA Relative Quantitation

High Accuracy and Precision

Standard curves demonstrate high accuracy (linearity with zero intercept) and precision (tight scatter around the line).

RNA Absolute Quantitation via HCR™ Northern Blot

Absolute Quantitation

Absolute quantitation is performed by comparing samples to a standard curve generated using RNA targets of known abundance.

Absolute miRNA Quantitation for HEK 293T and HeLa Cells

High Accuracy and Precision

Standard curves demonstrate high accuracy (linearity with zero intercept) and precision (tight scatter around the line).

Automatic Background Suppression

HCR™ Gold Northern Blots provide automatic background suppression throughout the protocol, ensuring that even if probes or amplifiers bind non-specifically in the sample they will not generate amplified background, dramatically enhancing performance and ease-of-use.

Straightforward Multiplexing

HCR™ Gold Northern Blots enable straightforward multiplexing using 1-step quantitative signal amplification for all target RNAs simultaneously.

Infinite Catalog

Whether your target is already in our Infinite Catalog or just joining it, HCR™ HiFi Probes for any target RNA in any organism across the tree of life are designed by the MI Team using MI’s proprietary probe design process with no design fee.

Quantitative Signal

HCR™ Gold Northern Blot signal is quantitative in the form of band intensities that scale approximately linearly with target abundance.

HCR RNA-FISH: Protocol Overview

The same 2-stage protocol is used independent of the number of target RNAs.

HCR™ Gold Northern Blots

Multiplex quantitative RNA blotting

Simple Robust Protocols

RNA Relative Quantitation via HCR™ Northern Blot

RNA Absolute Quantitation via HCR™ Northern Blot

Automatic Background Suppression

Straightforward Multiplexing

Infinite Catalog

Whether your target is already in our Infinite Catalog or just joining it, HCR™ HiFi Probes for any target RNA in any organism across the tree of life are designed by the MI Team using MI’s proprietary probe design process with no design fee.

Quantitative Signal

The same 2-stage protocol is used independent of the number of target RNAs.

HCR™ Gold Northern Blots

Multiplex quantitative RNA blotting

Simple Robust Protocols

RNA Relative Quantitation via HCR™ Northern Blot

RNA Absolute Quantitation via HCR™ Northern Blot

Automatic Background Suppression

Straightforward Multiplexing

Infinite Catalog

Whether your target is already in our Infinite Catalog or just joining it, HCR™ HiFi Probes for any target RNA in any organism across the tree of life are designed by the MI Team using MI’s proprietary probe design process with no design fee.​

Quantitative Signal

Whether your target is already in our Infinite Catalog or just joining it, HCR™ HiFi Probes for any target RNA in any organism across the tree of life are designed by the MI Team using MI’s proprietary probe design process with no design fee.