HCR™ Gold RNA-FISH
Multiplex, quantitative, high-resolution RNA imaging
HCR™ Gold RNA-FISH kits enable multiplex, quantitative, high-resolution RNA fluorescence in situ hybridization (RNA-FISH) with automatic background suppression throughout the protocol
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What comes in an HCR™ Gold RNA-FISH kit?
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HCR™ HiFi Probe
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HCR™ HiFi Probe Hybridization Buffer
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HCR™ HiFi Probe Wash Buffer
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HCR™ Gold Amplifier
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HCR™ Gold Amplifier Buffer
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HCR™ Gold Amplifier Wash Buffer
Simple Robust Protocols
HCR™ Gold RNA-FISH protocols are simple, robust, and enzyme-free, requiring only 2 stages (probe hybridization, amplification) independent of the number of target RNAs
Automatic Background Suppression
HCR™ Gold RNA-FISH kits provide automatic background suppression throughout the protocol, ensuring that even if probes or amplifiers bind non-specifically in the sample they will not generate amplified background, dramatically enhancing performance and ease-of-use.
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Straightforward Multiplexing
HCR™ Gold RNA-FISH enables straightforward multiplexing using 1-step quantitative signal amplification for 10 target RNAs simultaneously.
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Deep Sample Penetration
HCR™ Gold Amplifiers comprise small hairpins that penetrate thick samples (e.g., 1 cm whole-mount adult mouse brain) to execute an enzyme-free amplification cascade at the site of the target.
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Image Any Target mRNA. In Any Organism. In Any Sample Type.
HCR™ Gold RNA-FISH offers unmatched versatility (see gallery): FFPE tissue sections, fresh/fixed frozen tissue sections, thick brain slices, cells in solution or on a slide, whole-mounts, cleared samples, model organisms, non-model organisms, environmental samples, or multi-kingdom samples.
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Infinite Catalog
Whether your target is already in our Infinite Catalog or just joining it, HCR™ HiFi Probes for any target RNA in any organism across the tree of life are designed by the MI Team using MI’s proprietary probe design process with no design fee.​
Quantitative High-Resolution RNA Imaging
HCR™ Gold RNA-FISH enables quantification of RNA targets with subcellular and single-molecule resolution in an anatomical context using two complementary imaging modes (see subcellular/single-molecule comparison):
Subcellular Quantitative RNA Imaging
HCR™ Gold RNA-FISH enables analog RNA relative quantitation with subcellular resolution in the anatomical context of thick autofluorescent samples.
Single-molecule Quantitative RNA Imaging
HCR™ Gold RNA-FISH enables digital RNA absolute quantitation with single-molecule resolution in the anatomical context of thick autofluorescent samples.
Compatible with Tissue Clearing
HCR™ Gold RNA-FISH protocols are compatible with tissue clearing to reduce background in highly autofluorescent tissues.
Analog RNA relative quantitation with subcellular resolution in the anatomical context of thick autofluorescent samples
Digital RNA absolute quantitation with single-molecule resolution in the anatomical context of thick autofluorescent samples
Getting started?
For multiplex, quantitative, high-resolution HCR™ Gold RNA-FISH with automatic background suppression throughout the protocol:
✓ order one HCR™ Gold RNA-FISH kit per target RNA
✓ use a different HCR™ Gold Amplifier for each target RNA
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The same reagents can be used for multiplex quantitative HCR™ Gold RNA Flow Cytometry and HCR™ Gold Northern Blots with automatic background suppression throughout the protocol