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HCR™ Gold RNA-FISH/IF

Multiplex, quantitative, high-resolution RNA and protein imaging

HCR™ Gold RNA-FISH and HCR™ Gold IF kits enable a unified approach to multiplex, quantitative, high-resolution RNA fluorescence in situ hybridization (RNA-FISH) and immunofluorescence (IF)

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Simple Robust Protocols
HCR™ Gold RNA-FISH/IF protocols are simple, robust, and enzyme-free, requiring only 3 stages (probe binding to protein targets, probe hybridization to RNA targets, amplification) independent of the number of RNA and protein targets

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HCR™ Gold RNA-FISH/IF

1-step quantitative signal amplification for 10 RNA and protein targets simultaneously

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Straightforward Multiplexing

HCR™ Gold RNA-FISH/IF enables straightforward multiplexing using 1-step quantitative signal amplification for all RNA and protein targets simultaneously.

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Any organism across the tree of life

Free custom probe design for any target mRNA in any organism. For protein targets, plug-and-play using your own unmodified 1º antibodies of choice in combination with validated HCR™ 2º Antibody Probes.

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Simultaneous Quantitative High-Resolution RNA and Protein Imaging

HCR™ Gold RNA-FISH/IF enables RNA and protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.

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1-step quantitative HCR™ Gold signal amplification for 10 target RNAs and proteins simultaneously

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RNA and protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples

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Bidirectional quantitative RNA and protein discovery in an anatomical context

Getting started?

For multiplex quantitative HCR™ Gold RNA-FISH/IF: 

 âœ“ order one HCR™ Gold RNA-FISH kit per RNA target

 âœ“ order one HCR™ Gold IF kit per protein target 

 âœ“ use a different HCR™ Gold Amplifier for each target RNA or protein

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