HCR RNA-FISH: Protocol Overview
The same 2-stage protocol is used independent of the number of target RNAs.
All major
automation platforms
HCR™ IF Technology
Multiplexed, quantitative, high-resolution protein imaging
HCR™ probes, amplifiers, and buffers enable multiplexed, quantitative, high-resolution immunofluorescence (IF), immunohistochemistry (IHC), immunocytochemistry (ICC), and immunostaining.
HCR™ IF: How It Works
Detection Stage
Amplification Stage
Detection Stage
Protein targets are detected with unlabeled primary antibody probes and HCR™ probe comprising an initiator-labeled secondary antibody.
Amplification Stage
An HCR™ amplifier comprises metastable fluorescent hairpins h1 and h2. Initiator i1 triggers self-assembly of tethered fluorescent amplification polymers.
Experience Multiplexed, Quantitative, High-Resolution
Immunofluorescence
Amplified. Enzyme-Free. FFPE. Simple.
Simple Robust Protocols
HCR™ IF protocols are simple, robust, and enzyme-free, requiring only 2 stages independent of the number of target proteins.
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Straightforward Multiplexing
HCR™ enables straightforward multiplexing using 1-step quantitative signal amplification for all target proteins simultaneously.
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Plug-and-Play with Your 1º Antibodies of Choice
For protein targets in any organism across the tree of life, plug-and-play using your own 1º antibodies of choice (without modification) in combination with validated HCR™ 2º antibody probes.
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HCR™ Protein-IF + HCR™ RNA-FISH
Combine HCR™ IF probes with HCR™ RNA-FISH probes to enable HCR™ protein-IF + HCR™ RNA-FISH for multiplexed, quantitative, high-resolution protein and RNA imaging with 1-step HCR™ signal amplification for all protein and RNA targets simultaneously.
Subcellular Quantitative Protein Imaging
Subcellular protein imaging enables protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.
The same 2-stage enzyme-free protocol is used independent of the number of target proteins
Protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples
