Straightforward Multiplexing
Straightforward multiplexing using 10 orthogonal HCR™ Gold Amplifiers
with 1-step, quantitative, high-gain, enzyme-free signal amplification for all RNA and/or protein targets simultaneously
Use in your assay format of choice:
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HCR™ Gold RNA-FISH (RNA fluorescence in situ hybridization)
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HCR™ Gold IF (immunofluorescence)
4-Plex HCR™ Gold RNA-FISH/IF with Bandpass Imaging
We recommend labels (488, 546, 647, 750) for robust 4-plex bandpass imaging.
10-Plex HCR™ Gold RNA-FISH/IF with Spectral Imaging and Linear Unmixing
We recommend labels (405, 425, 488, 514, 546, 594, 633, 700, 750, 800) for robust 10-plex spectral imaging. Linear unmixing yields one fluorescent channel per fluorophore plus one channel for autofluorescence.
A new era for in situ hybridization:
multiplex, quantitative, high-resolution, sensitive, versatile, robust
Experience multiplex, quantitative, high-resolution immunfluorescence: amplified, enzyme-free, FFPE, simple
Quantitative 1-step enzyme-free HCR™ Gold signal amplification for all RNA and protein targets simultaneously